Xenopus oocytes are a versatile expression system particularly suited for membrane transporters and channels. The vocal apparatus of the clawed frog is designed for underwater sound production (Deuchar, 1975). Physicochemical Properties of Oocytes, Springer Nature is developing a new tool to find and evaluate Protocols. Microinjection of size-fractionated mRNA from the tissue sources, followed by subsequent functional assays, narrows down the potential target mRNAs to a relatively smaller subpopulation. This procedure does not affect the electrophysiological responses of the oocytes (measured at least 36 hr later) or in any way alter its ability to translate ion channel mRNA (reviewed in Ref. Interestingly, systematists now consider X. tropicalis and X. epitropicalis to warrant placement into their own genus (Silurana) on the basis of morphological characteristics (e.g., Cannatella and Trueb, 1988; de Sá and Hillis, 1990; Cannatella and de Sá, 1993; Rödel, 2000; Frost, 2004). Microinjection of Xenopus Laevis Oocytes Article doi: 10.3791/1106. 5 ). Xenopus Laevis Oocytes, supplied by Integrated DNA Technologies, used in various techniques. In captivity, the clawed frog can live 15 years or more (Deuchar, 1975; Tinsley et al., 1996). Xenopus laevis oocytes possess the complete set of translational machinery (e.g., ribosomes, enzymes, transfer RNAs, and translation cofactors) for subsequent use in early embryonic development. Figure 2: Xenopus laevis oocytes before and after maturation. Each region was analyzed to determine the amount of total cellular RNA, poly(A)+RNA, and newly synthesized ribosomal RNA. Stage I consists of small (50 to 100 μ) colorless oocytes whose cytoplasm is transparent. BACKGROUND. Immature eggs (oocytes) of the South African clawed frog Xenopus laevis are considered an ideal expression system for such studies. Barnard, E. A., Miledi, R., and Sumikawa, K. (1982) Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes. Oviductin is inhibited by DFP, aprotinin, soybean trypsin inhibitor, leupeptin, antipain EDTA, and EGTA, and also weakly by TLCK, TPCK, and 1,10-phenanthroline; pepstatin, E-64, and iodoacetamide do not inhibit [5]. Xenopus Laevis Oocytes, supplied by Integrated DNA Technologies, used in various techniques. Furthermore, when MPF is injected into recipient oocytes, it is subject to an “auto-amplification” process, since it retains its full activity after up to 10 serial transfers of cytoplasm into untreated oocytes (7,9). Ecocyte Bioscience. Oogenesis in the anuran Xenopus laevis can be divided into six stages based on the anatomy of the developing oocyte. To obtain good material during the critical northern hemisphere summer season, we induce ovulation in early spring by injecting into the dorsal lymph sac first 50 IU and then, after a 6-hr interval, 350 IU/female of human chorionic gonadotropin [CG 5, Sigma (St. Louis, MO); or Pregnyl, Organon (Oss, The Netherlands), at 500 IU/ml in distilled water]. (2001) Inhibition of the glutamate transporter EAAC1 expressed in. Their large nuclei and mitochondrial masses are clearly visible in the intact oocyte. A schematic representation is shown. Furthermore, several pharmacological membrane active agents were able to mimick progesterone action. BACKGROUND. Not logged in Bioz Stars score: 90/100, based on 1 PubMed citations. The open state has a … Bioz Stars score: 90/100, based on 1 PubMed citations. (1993) Receptors that couple to 2 classes of G proteins increase cAMP and activate CFTR expressed in, Landau, E. M. and Blitzer, R. D. (1994) Chloride current assay for phospholipase C in, Guo, Z., Liliom, K., Fischer, D. J., et al. [18] Although X. laevis does not have the short generation time and genetic simplicity generally desired in genetic model organisms , it is an important model organism in developmental biology , cell biology , toxicology and neurobiology . D. Bertrand, ... M. Ballivet, in Methods in Neurosciences, 1991. Please Specify Uncurling arrive around 38 NF
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